| Peer-Reviewed

Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste

Received: 15 May 2013     Published: 10 July 2013
Views:       Downloads:
Abstract

The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.

Published in International Journal of Renewable and Sustainable Energy (Volume 2, Issue 4)
DOI 10.11648/j.ijrse.20130204.11
Page(s) 133-139
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Ethanol, Molassesand Stress Tolerant

References
[1] Jones, A.M., K.C. Thomas and W.M. Inglew, Ethanolic fermentation of molasses and sugarcane juice using very high gravity technology. Journal of Agricultural Chemistry, 1994. 42: p. 1242-12 46.
[2] Isaias, M., V. Leal, L.M. Ramos and J.A. Da-Silva, Assessment of greenhouse gas emissions in the production and use of fuel ethanol in Brazil.Secretariat of the Environment, Government of the State of Sao Paulo, 2004.
[3] Goettemoeller, J. and A. Goettemoeller, Sustainable Ethanol: Biofuels, Biorefineries, Cellulosic Biomass, Flex-Fuel Vehicles, and Sustainable Farming for Energy Independence. Praire Oak Publishing, Maryville, Missouri, ISBN: 9780978629304, 2007. p. 42.
[4] Chandel, A.K., E.S. Chan, R. Rudravaram, M.L. Narasu, L.V. Rao and P. Ravindra, Economics and Enviromental Impact of Bioethanol Production Technologies: an Appraisal. Biotechnology and Molecular Biology Review, 2007. 2(1): p. 14-32.
[5] Tsuyoshi, N., R. Fudou, S. Yamanaka, M. Kozaki, N. Tamang, S. Thapa, J.P. Tamang, Identification of yeast strains isolated from marcha in Sikkim, a microbial starter for amylolytic fermentation.International Journal of Food Microbiology, 2005. 9(2): p. 135-146.
[6] Osunkoya, O.A. and N.J. Okwudinka, Utilization of sugar refinery waste (molasses) for ethanol productionusing Saccharomyces cerevisiae.American Journal of Scientific and Industrial Research, 2011. 2(4): p. 694-706.
[7] Somda, M.K., A. Savadogo, C.A.T. Ouattara, A.S. Ouattara and A.S. Traore, Thermotolerant and Alcohol-tolerant yeasts targeted to optimize hydrolyzation from mango peel for high bioethanol production. Asian Journal of Biotechnology, 2011. 3 (1): p. 77-83.
[8] Khongsay, N., L. Laopaiboon and P. Laopaiboon, Growth and Batch fermentation of Saccharomyces cerevisiae on sweet sorghum stem juice under normal and very high gravity conditions. Biotechnology, 2010. 9(1): p. 9-16.
[9] Boboye, B. andI. Dayo-Owoyemi, Evaluation of Dough Sensory Properties Impacted by Yeasts Isolated from Cassava. Journal of Applied Sciences, 2009. 9(4): p. 771-776.
[10] Fakruddin, M., M.A. Quayum, M.M. Ahmed, N. Chowdhury, Analysis of Key Factors Affecting Ethanol Production by Saccharomyces cerevisiae IFST-072011. Biotechnology, 2012. 11(4): p. 248-252.
[11] Miller, G.L., Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Analytical Chemistry, 1959. 31: p. 426-28.
[12] Conway, E.J., Microdiffusion analysis and volumetric error. Crosby Lockwood and Son, London, 1939.
[13] Lodder, J., The yeasts: A Taxonomic study. NorthHoll and Publishing, Amsterdam, 1971.
[14] Boekhout, T. and C.P. Kurtzman, Principles and methods used in yeast classification, and an overview of currently accepted yeast genera. In Wolf, K. Nonconventional Yeasts in Biotechnology: A Handbook. Springer-Verlag: Heidelberg. 1996. p. 1-99
[15] Mariam, I., K. Manzoor, S. Ali and Ikram-Ul-Haq, Enhanced production of from free and immobilized Saccharomyces cerevisiaeunder stationary culture. Pakistan Journal of Botany, 2009. 41: p. 821823.
[16] Arshad, M., M.A. Zia, M. Asghar and H. Bhatti, (2011). Improving bio-ethanol yield: Using virginiamycin and sodium flouride at a Pakistani distillery. African Journal of Biotechnology, 2011. 10(53): p. 11071-11074.
[17] Willaert, R. and A.N. Viktor, Primay beer fermentation by immobilized yeast - a review on flavor formation and control strategies. Journal of Chemical Technology & Biotechnology, 2006. 81: p. 1353-1367.
[18] Walker, G.M. and P.V. Dijck, Physiological and molecular responses of yeasts to the environment, p. 111-152. In Querol, A. and G. Fleet (eds.), Yeasts in food and beverages, Springer-Verlag Berlin, Germany, 2006.
Cite This Article
  • APA Style

    Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. (2013). Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. International Journal of Sustainable and Green Energy, 2(4), 133-139. https://doi.org/10.11648/j.ijrse.20130204.11

    Copy | Download

    ACS Style

    Md. Fakruddin; Md. Ariful Islam; Monzur Morshed Ahmed; Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int. J. Sustain. Green Energy 2013, 2(4), 133-139. doi: 10.11648/j.ijrse.20130204.11

    Copy | Download

    AMA Style

    Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int J Sustain Green Energy. 2013;2(4):133-139. doi: 10.11648/j.ijrse.20130204.11

    Copy | Download

  • @article{10.11648/j.ijrse.20130204.11,
      author = {Md. Fakruddin and Md. Ariful Islam and Monzur Morshed Ahmed and Nayuum Chowdhury},
      title = {Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste},
      journal = {International Journal of Sustainable and Green Energy},
      volume = {2},
      number = {4},
      pages = {133-139},
      doi = {10.11648/j.ijrse.20130204.11},
      url = {https://doi.org/10.11648/j.ijrse.20130204.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.20130204.11},
      abstract = {The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.},
     year = {2013}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste
    AU  - Md. Fakruddin
    AU  - Md. Ariful Islam
    AU  - Monzur Morshed Ahmed
    AU  - Nayuum Chowdhury
    Y1  - 2013/07/10
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ijrse.20130204.11
    DO  - 10.11648/j.ijrse.20130204.11
    T2  - International Journal of Sustainable and Green Energy
    JF  - International Journal of Sustainable and Green Energy
    JO  - International Journal of Sustainable and Green Energy
    SP  - 133
    EP  - 139
    PB  - Science Publishing Group
    SN  - 2575-1549
    UR  - https://doi.org/10.11648/j.ijrse.20130204.11
    AB  - The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.
    VL  - 2
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial research (BCSIR), Dhaka, Bangladesh

  • Departments of Biotechnology, BRAC University, Dhaka, Bangladesh

  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial research (BCSIR), Dhaka, Bangladesh

  • Departments of Biotechnology, BRAC University, Dhaka, Bangladesh

  • Sections